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unique access array barcoding primers  (fluidigm)


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    Structured Review

    fluidigm unique access array barcoding primers
    Unique Access Array Barcoding Primers, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 843 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/access+array+barcode+primers/Access+Array/bio_rxiv__64898__2026__01__29__702624-274-0-5
    Average 93 stars, based on 843 article reviews
    unique access array barcoding primers - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Amplification:

    Article Title: Shedding light on the total and active core microbiomes in slow sand filters for drinking water production.
    Article Snippet: PCR amplifications were performed using the Taq Master Mix (QIAGEN, USA).The first stage of PCR amplification protocol was 95 ◦C for 5 min followed by 25 cycles of 95 ◦C for 30 s, 55 ◦C for 30 s and 72 ◦C for 1 min, with the final elongation step at 72 ◦C for 7 min. PCR products were checked on a 2% (w/v) agarose gel to determine the success of amplification. .. Subsequently, the second amplification step was performed using 2 μl of the first-stage PCR product and Access Array barcode primers (Fluidigm, USA) for 8 cycles to generate the second-stage PCR products in 20 μl reaction volumes. .. The second-stage PCR products were examined on a 2% (w/v) agarose gel and then sent for sequencing on the Illumina Miseq platform.

    Polymerase Chain Reaction:

    Article Title: Shedding light on the total and active core microbiomes in slow sand filters for drinking water production.
    Article Snippet: PCR amplifications were performed using the Taq Master Mix (QIAGEN, USA).The first stage of PCR amplification protocol was 95 ◦C for 5 min followed by 25 cycles of 95 ◦C for 30 s, 55 ◦C for 30 s and 72 ◦C for 1 min, with the final elongation step at 72 ◦C for 7 min. PCR products were checked on a 2% (w/v) agarose gel to determine the success of amplification. .. Subsequently, the second amplification step was performed using 2 μl of the first-stage PCR product and Access Array barcode primers (Fluidigm, USA) for 8 cycles to generate the second-stage PCR products in 20 μl reaction volumes. .. The second-stage PCR products were examined on a 2% (w/v) agarose gel and then sent for sequencing on the Illumina Miseq platform.

    Article Title: PCR effects of melting temperature adjustment of individual primers in degenerate primer pools
    Article Snippet: During the second stage PCR amplification, both Fluidigm Access Array Barcode and the ShortEMP primers were included, and 12 cycles of amplification were performed in place of eight. .. In this approach, common sequences are incorporated during the second stage PCR as the ShortEMP primers with linkers amplify the NoLinker_ShortEMP amplicons; subsequently, the Fluidigm Access Array barcode primers amplify amplicons containing the common sequence linkers. .. In all experiments, samples were pooled using an EpMotion5075 liquid handling robot (Eppendorf, Hamburg, Germany).

    Sequencing:

    Article Title: PCR effects of melting temperature adjustment of individual primers in degenerate primer pools
    Article Snippet: During the second stage PCR amplification, both Fluidigm Access Array Barcode and the ShortEMP primers were included, and 12 cycles of amplification were performed in place of eight. .. In this approach, common sequences are incorporated during the second stage PCR as the ShortEMP primers with linkers amplify the NoLinker_ShortEMP amplicons; subsequently, the Fluidigm Access Array barcode primers amplify amplicons containing the common sequence linkers. .. In all experiments, samples were pooled using an EpMotion5075 liquid handling robot (Eppendorf, Hamburg, Germany).



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    CS1, common sequence 1 linker sequence. CS2, common sequence 2 linker sequence. BC, <t>barcode.</t> F, Forward primer. R, Reverse primer, P5/P7, <t>Illumina</t> primers, PE1/PE2, Fluidigm Access Array Barcode Library Illumina adapters. In stage 1 (linear copying only), individual samples are cycled for four cycles with locus-specific primers and Fluidigm barcoded primers. Subsequently, all reactions are pooled and purified together, and then amplified with Illumina P5 and P7 primers in stage 2 (exponential amplification with primers targeting linker sequences). During stage 1, linear copying of templates leads to products which contain Illumina sequencing adapters, sample-specific barcodes, and the locus-specific region of interest. Only fragments with Illumina adapters and barcodes are exponentially amplified in stage 2. Locus-specific primer sequences can be modified as needed.
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    Image Search Results


    CS1, common sequence 1 linker sequence. CS2, common sequence 2 linker sequence. BC, barcode. F, Forward primer. R, Reverse primer, P5/P7, Illumina primers, PE1/PE2, Fluidigm Access Array Barcode Library Illumina adapters. In stage 1 (linear copying only), individual samples are cycled for four cycles with locus-specific primers and Fluidigm barcoded primers. Subsequently, all reactions are pooled and purified together, and then amplified with Illumina P5 and P7 primers in stage 2 (exponential amplification with primers targeting linker sequences). During stage 1, linear copying of templates leads to products which contain Illumina sequencing adapters, sample-specific barcodes, and the locus-specific region of interest. Only fragments with Illumina adapters and barcodes are exponentially amplified in stage 2. Locus-specific primer sequences can be modified as needed.

    Journal: PeerJ

    Article Title: Quantitating primer-template interactions using deconstructed PCR

    doi: 10.7717/peerj.17787

    Figure Lengend Snippet: CS1, common sequence 1 linker sequence. CS2, common sequence 2 linker sequence. BC, barcode. F, Forward primer. R, Reverse primer, P5/P7, Illumina primers, PE1/PE2, Fluidigm Access Array Barcode Library Illumina adapters. In stage 1 (linear copying only), individual samples are cycled for four cycles with locus-specific primers and Fluidigm barcoded primers. Subsequently, all reactions are pooled and purified together, and then amplified with Illumina P5 and P7 primers in stage 2 (exponential amplification with primers targeting linker sequences). During stage 1, linear copying of templates leads to products which contain Illumina sequencing adapters, sample-specific barcodes, and the locus-specific region of interest. Only fragments with Illumina adapters and barcodes are exponentially amplified in stage 2. Locus-specific primer sequences can be modified as needed.

    Article Snippet: A master mix for the entire plate was made using the MT master mix, and each well received a separate primer pair with a unique Access Array Barcode Library for Illumina primer (described above).

    Techniques: Sequencing, Purification, Amplification, Illumina Sequencing, Modification

    CS1, common sequence 1 linker sequence. CS2, common sequence 2 linker sequence. BC, barcode. F, Forward primer. R, Reverse primer, P5/P7, Illumina primers, PE1/PE2, Fluidigm Access Array Barcode Library Illumina adapters. In stage 1 (linear copying only), individual samples are cycled for four cycles with locus-specific primers and Fluidigm barcoded primers. Subsequently, all reactions are pooled and purified together, and then amplified with Illumina P5 and P7 primers in stage 2 (exponential amplification with primers targeting linker sequences). During stage 1, linear copying of templates leads to products which contain Illumina sequencing adapters, sample-specific barcodes, and the locus-specific region of interest. Only fragments with Illumina adapters and barcodes are exponentially amplified in stage 2. Locus-specific primer sequences can be modified as needed.

    Journal: PeerJ

    Article Title: Quantitating primer-template interactions using deconstructed PCR

    doi: 10.7717/peerj.17787

    Figure Lengend Snippet: CS1, common sequence 1 linker sequence. CS2, common sequence 2 linker sequence. BC, barcode. F, Forward primer. R, Reverse primer, P5/P7, Illumina primers, PE1/PE2, Fluidigm Access Array Barcode Library Illumina adapters. In stage 1 (linear copying only), individual samples are cycled for four cycles with locus-specific primers and Fluidigm barcoded primers. Subsequently, all reactions are pooled and purified together, and then amplified with Illumina P5 and P7 primers in stage 2 (exponential amplification with primers targeting linker sequences). During stage 1, linear copying of templates leads to products which contain Illumina sequencing adapters, sample-specific barcodes, and the locus-specific region of interest. Only fragments with Illumina adapters and barcodes are exponentially amplified in stage 2. Locus-specific primer sequences can be modified as needed.

    Article Snippet: For barcoding reactions, Access Array Barcode Library for Illumina primers were purchased (Fluidigm, South San Francisco, CA, USA).

    Techniques: Sequencing, Purification, Amplification, Modification